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Molecular identification of weedy Sporobolus species by PCR‐RFLP
Author(s) -
SHRESTHA S,
GRAHAM G C,
LOCH D S,
ADKINS S W
Publication year - 2010
Publication title -
weed research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 74
eISSN - 1365-3180
pISSN - 0043-1737
DOI - 10.1111/j.1365-3180.2010.00806.x
Subject(s) - noxious weed , biology , restriction fragment length polymorphism , botany , internal transcribed spacer , ribosomal dna , restriction enzyme , species identification , polymerase chain reaction , weed , ribosomal rna , zoology , genetics , gene , phylogenetics
Shrestha S, Graham GC, Loch DS & Adkins SW (2010). Molecular identification of weedy Sporobolus species by PCR‐RFLP. Weed Research. 50 , 383–387. Summary Identification of the five noxious weedy Sporobolus species from non‐weedy and native species in Australia is extremely difficult. Furthermore, seeds of these species, which are found in commercial seed lots, are morphologically indistinguishable. The lack of a reliable identification protocol is contributing to the rapid spread of these noxious weedy species within Australia. A polymerase chain reaction‐restriction fragment length polymorphism approach carried out on nuclear ribosomal DNA (rDNA) internal transcribed spacer (ITS) sequences was used to create a molecular diagnostic tool. To develop this tool, 40 ITS sequences generated from 14 Sporobolus species were used. A restriction enzyme ( Mvn I) was identified which could cut the ITS sequences from the noxious weedy species into two approximately equal fragments, while not cutting those from all other species. When tested on 23 randomly selected seed samples, identification was accomplished with 100% accuracy, suggesting a possible application in seed certification programmes.