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Blue light induced changes in inositol 1,4,5‐trisphosphate in Cuscuta campestris seedlings
Author(s) -
HAIDAR M A,
BOSS W F
Publication year - 2009
Publication title -
weed research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 74
eISSN - 1365-3180
pISSN - 0043-1737
DOI - 10.1111/j.1365-3180.2009.00732.x
Subject(s) - blue light , cuscuta , inositol , inositol trisphosphate , second messenger system , pertussis toxin , chemistry , receptor , biophysics , signal transduction , biology , microbiology and biotechnology , g protein , botany , biochemistry , optics , physics
Summary Previous studies revealed that blue light stimulates and red light inhibits prehaustoria development in young seedlings of the parasitic weed Cuscuta campestris (field dodder). This study showed a positive correlation between blue light induced increases in inositol 1,4,5‐trisphosphate (IP 3 ) and blue light‐mediation of prehaustoria development, prior to host attachment. Blue light induced a significant increase in the level of IP 3 , with a peak at about 30 min. Thereafter, the level of IP 3 declined to the resting value after 2 h of blue light. Irradiation with 10 min red light pulse applied directly at the end of each 0.05, 0.1, 0.15, 0.2, 0.3, 1, 2 and 4 h blue light significantly reduced IP 3 , while high levels of IP 3 were observed after 10 min far‐red pulse. The G‐protein inhibitor pertussis toxin inhibited prehaustoria developed under blue light, suggesting that receptor‐coupled G‐proteins are likely to be involved in prehaustoria development. These results are the first in vivo demonstration of a possible role for IP 3 as a second messenger in the blue light signal transduction process in prehaustoria development in Cuscuta .