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Genome‐wide microarray analysis of the effect of a surfactant application in Arabidopsis
Author(s) -
MADHOU P,
RAGHAVAN C,
WELLS A,
STEVENSON T W
Publication year - 2006
Publication title -
weed research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 74
eISSN - 1365-3180
pISSN - 0043-1737
DOI - 10.1111/j.1365-3180.2006.00511.x
Subject(s) - jasmonic acid , gene , microarray analysis techniques , gene expression , arabidopsis , biochemistry , cytochrome p450 , biology , detoxification (alternative medicine) , microarray , downregulation and upregulation , enzyme , chemistry , mutant , medicine , alternative medicine , pathology
Summary Herbicides provide growers with the means to chemically manage weeds; surfactants are often an effective way of improving the performance of these herbicides. However, the mode of action of surfactants at the molecular level is not well understood. As a preliminary step towards understanding the molecular mechanism of action of an etheramine surfactant, plant gene expression in response to foliar application of surfactant NUL1026 was analysed in Arabidopsis thaliana , using the commercially available Affymetrix ATH1‐121501 chip. One hundred and ninety‐six genes were found to be significantly altered 1 h after plants were treated with 0.2% ( V / V ) of surfactant NUL1026. Functional category analysis of these genes revealed that the largest categories included metabolism, physiological processes, transport, protein metabolism, response to stimulus and transcription. A number of genes encoding detoxification proteins, such as cytochrome P450, glutathione S ‐transferase and members of the multidrug‐resistance associated proteins, were upregulated. Interestingly, microarray results showed a number of genes encoding enzymes involved in the jasmonic acid biosynthesis pathway and also the 1‐aminocyclopropane‐1‐carboxylate synthase gene for ethylene production were upregulated, indicating that treatment with surfactant NUL1026 affects the expression of a number of genes involved in the detoxification and signalling pathways.