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Rapid detection of‘triazine resistant’weeds using chlorophyll fluorescence
Author(s) -
ALI A.,
MACHADO V. SOUZA
Publication year - 1981
Publication title -
weed research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 74
eISSN - 1365-3180
pISSN - 0043-1737
DOI - 10.1111/j.1365-3180.1981.tb00116.x
Subject(s) - fluorometer , triazine , chlorophyll fluorescence , fluorescence , herbicide resistance , chlorophyll , chlorophyll a , chemistry , biology , botany , agronomy , horticulture , weed , optics , physics , polymer chemistry
Summary: A simple and rapid method for the detection of triazine resistant plants is reported, based on chlorophyll fluorescence. Leaf discs were floated in phosphate buffer solution with and without 10 −4 M alrazine for 2–4 h. Subsequently, the relative leaf chlorophyll fluorescence (LCF) was measured with a plant productivity fluorometer. Following herbicide uptake LCF in triazine susceptible plants was 2–3 times greater than in the untreated controls; no such LCF changes were observed in triazine resistant plants. Concentration and time course studies were conducted on several species having triazine susceptible and resistant biotypes. The use of this method for field extension purposes is described.