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Inhibition of photosynthetic pigments in cucumber cotyledons as a principle for a bioassay with fluridone
Author(s) -
And D.M. DREXLER,
FLETCHER R.A.
Publication year - 1981
Publication title -
weed research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 74
eISSN - 1365-3180
pISSN - 0043-1737
DOI - 10.1111/j.1365-3180.1981.tb00098.x
Subject(s) - fluridone , bioassay , chemistry , botany , photosynthesis , pigment , light intensity , cucumis , carotenoid , etiolation , xanthophyll , horticulture , biology , biochemistry , ecology , organic chemistry , optics , physics , enzyme , abscisic acid , gene
Summary: The response of etiolated cucumber ( Cucumis sativus L. cv. National Pickling) cotyledons to fluridone was investigated in terms of inhibition of photosynthetic pigments, and utility as a bioassay. At a light intensity of 35 W/m 2 , chlorophyll levels were higher in the controls, than at either 12.9 W/m 2 , or 75 W/m 2 . Fluridone inhibition was also more pronounced at that intensity. Significant differences between treatments were established as early as 24 h after exposure to light, when at the lowest fluridone concentration of 0.001 μg/ml, chlorophylls and carotenoids were inhibited 25 and 35% respectively. The system's simplicity and sensitivity suggest its use as a bioassay. It is shown that simple monochromatic absorbances of acetone extracts can satisfactorily estimate chlorophylls and carotenoids. Concentrations of fluridone as minute as 10 −9 moles/ litre are easily delected, and the system could feasibly detect even lower levels.