Premium
A RAPID BIOASSAY FOR SIMULTANEOUS IDENTIFICATION AND QUANTITATJON OF PICLORAM IN AQUEOUS SOLUTION
Author(s) -
REID C. P. P.,
HURTT W.
Publication year - 1969
Publication title -
weed research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 74
eISSN - 1365-3180
pISSN - 0043-1737
DOI - 10.1111/j.1365-3180.1969.tb01462.x
Subject(s) - picloram , bioassay , lactuca , chromatography , chemistry , hypocotyl , botany , biology , genetics
Summary. When seeds of lettuce ( Lactuca sativa L.) were placed on small squares of filter paper moistened with solutions of picloram (4‐amino‐3,5,6‐trichloropicolinic acid), the degree of inhibition on of root and hypocotyl elongation 72 hr later was related to picloram concentration. Inhibition was a useful parameter in determining quantities of picloram ranging from 0·03 to 7·2 μg. (Growth stimulation occurred from 0·00072 to 0·0072 μg. After paper chromatography of plant exudates containing 14 C‐labelled picloram, location and quantities of picloram on the chromatograms were determined by the lettuce bioassay and compared with determinations by the methods of ultraviolet light sensitivity, 14 C 4π strip scanning and 14 C dilution calculations. R f values determined lay all methods were identical, and the quantitative determination by the bioassay agreed closely with the calculations based on the amount of 14 C detected by liquid scintillation counting. Un essai biologique rapide d'idntification et de dosage de piclorame en solution aqueuse