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In vitro antiseptic susceptibilities for Staphylococcus pseudintermedius isolated from canine superficial pyoderma in Japan
Author(s) -
Murayama Nobuo,
Nagata Masahiko,
Terada Yuri,
Okuaki Mio,
Takemura Noriyuki,
Nakaminami Hidemasa,
Noguchi Norihisa
Publication year - 2013
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/j.1365-3164.2012.01103.x
Subject(s) - staphylococcus pseudintermedius , microbiology and biotechnology , antiseptic , chlorhexidine , efflux , acriflavine , minimum inhibitory concentration , pyoderma , multiple drug resistance , staphylococcus , medicine , antibiotics , staphylococcus aureus , chemistry , biology , bacteria , dermatology , dentistry , pathology , biochemistry , genetics
Background – Topical therapy, particularly with chlorhexidine, is becoming increasingly common as a treatment option for canine pyoderma; however, there are limited studies on the susceptibility of Staphylococcus pseudintermedius to chlorhexidine compounds. Objectives – To determine the in vitro susceptibility of both meticillin‐resistant and meticillin‐susceptible S. pseudintermedius isolates to chlorhexidine and other antiseptic agents and the presence of multidrug efflux pump genes. Samples – One hundred S. pseudintermedius isolates from 23 initial and 77 recurrent cases of canine pyoderma. Methods – After bacterial identification and mecA testing, minimal inhibitory concentrations (MICs) of antiseptic agents were determined. Multidrug efflux pump genes, including qacA , qacB and smr , were identified. Results – Of the 100 isolates, 57 were identified as meticillin‐resistant S. pseudintermedius . The MIC 90 of chlorhexidine acetate, chlorhexidine gluconate, acriflavine, ethidium bromide and benzalkonium chloride were 1, 1, 2, 0.5 and 2 μg/mL, respectively. Multidrug efflux pump genes qacA , qacB and smr were not detected in any of the isolates. Conclusions and clinical importance – The MICs for chlorhexidine and other antiseptics remain low, and multidrug efflux pump genes were not found in the tested isolates.