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Effect of recombinant canine interferon‐γ on granulocyte–macrophage colony‐stimulating factor, transforming growth factor‐β and CC chemokine ligand 17 mRNA transcription in a canine keratinocyte cell line (CPEK)
Author(s) -
Shibata Sanae,
Maeda Sadatoshi,
Kondo Naho,
Inoue Akiko,
Maeda Shingo,
Chimura Naoki,
Fukata Tsuneo
Publication year - 2011
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/j.1365-3164.2010.00897.x
Subject(s) - chemokine , cytokine , granulocyte macrophage colony stimulating factor , messenger rna , microbiology and biotechnology , transcription factor , immunology , cell culture , biology , cancer research , inflammation , gene , biochemistry , genetics
Recombinant canine interferon‐γ (rCaIFN‐γ) produced by a baculovirus expression system has therapeutic efficacy against atopic dermatitis in dogs. Although the mechanism of action of rCaIFN‐γ is not completely understood, rCaIFN‐γ is thought to downregulate the activity of interleukin‐4‐ and interleukin‐5‐producing T helper 2 cells. However, rCaIFN‐γ may also act directly on canine keratinocytes by inhibiting the release of inflammatory mediators. In this study, we investigated the effects of rCaIFN‐γ on cytokine and chemokine mRNA transcription in a canine keratinocyte cell line, CPEK. It was found that granulocyte–macrophage colony‐stimulating factor (GM‐CSF) mRNA transcription was significantly inhibited after treatment with rCaIFN‐γ ( P < 0.001), whereas transforming growth factor‐β and CC chemokine ligand 17 mRNA levels were unchanged. This study suggests that rCaIFN‐γ may suppress GM‐CSF production from canine keratinocytes, although further studies are required to confirm this.