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Effect of a novel topical diester glucocorticoid spray on immediate‐ and late‐phase cutaneous allergic reactions in Maltese–beagle atopic dogs: a placebo‐controlled study
Author(s) -
Bizikova Petra,
Linder Keith E.,
Paps Judy,
Olivry Thierry
Publication year - 2010
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/j.1365-3164.2009.00782.x
Subject(s) - beagle , maltese , medicine , placebo , glucocorticoid , dermatology , anesthesia , pathology , philosophy , linguistics , alternative medicine
The inhibitory effect of 0.0584% hydrocortisone aceponate spray on immediate‐ and late‐phase skin reactions and the duration of inhibition after medication withdrawal were studied in 10 Maltese‐beagle atopic dogs. All subjects were sprayed on axillary and inguinal regions and on one randomly chosen side of the thorax once daily for 14 (phase 1) or 7 days (phase 2). Intradermal injections (IDT) of histamine and anticanine IgE antiserum were performed bilaterally on the thorax before, 7 and 14 days after treatment. During phase 2, IDT was performed once weekly for 5 weeks. Each IDT was evaluated by an investigator blinded to the site of active treatment. Skin biopsies of 24‐h anti‐IgE‐associated late‐phase reactions were collected from both thoracic sides before and 14 days after treatment to determine the number of inflammatory cells and dermal thickness. Phase 1 : Histamine and anti‐IgE‐induced global wheal scores at treated sites were significantly lower after 7 and 14 days with negative reactions present in >90% of dogs. Late‐phase reactions at both sides were also significantly decreased compared with that at baseline, and this was associated with reduced inflammatory cell influx. Moreover, a significant decrease in dermal thickness was recorded at treated sides after 14 days. Phase 2 : Histamine reactions became positive at untreated sides in all dogs 2 weeks after treatment. In conclusion, the 0.0584% hydrocortisone aceponate spray significantly decreased immediate‐ and late‐phase IDT reactions, and prolonged application caused skin atrophy at treated sites. A 2‐week withdrawal period prior to IDT is proposed.