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Rapid detection of Arthroderma vanbreuseghemii in rabbit skin specimens by PCR–RFLP
Author(s) -
Arabatzis Michael,
Xylouri Eftihia,
Frangiadaki Irene,
Tzimogianni Aleka,
Milioni Afroditi,
Arsenis George,
Velegraki Aristea
Publication year - 2006
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/j.1365-3164.2006.00535.x
Subject(s) - restriction fragment length polymorphism , rabbit (cipher) , biology , medicine , microbiology and biotechnology , polymerase chain reaction , genetics , gene , computer science , computer security
Three commercial rabbit farms independently reported suspected dermatophyte infections during 2004. Cultures confirmed that the infection was caused by the zoophilic Tricophyton mentagrophytes, while mating studies and sequencing of the internal transcribed spacer (ITS)1/4 polymerase chain reaction products, directly amplified from skin lesions and from pure culture isolates, established its Arthroderma vanbreuseghemii lineage. Subsequently, the corresponding Dde I restriction profiles of the ITS1/4 PCR products matched one of the two published T . mentagrophytes complex profiles. Sequence analysis of the published ITS sequences predicted distinct Dde I restriction sites for Arthroderma benhamiae and A. vanbreuseghemii , while all Dde I‐generated restriction fragment length polymorphisms of the rabbit isolates were identical to those of A. vanbreuseghemii. The proposed procedure can be used for rapid dermatophyte detection and presumptive T. mentagrophytes identification.