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Mouse epidermal development: effects of retinoic acid exposure in utero
Author(s) -
GARCÍAFERNÁNDEZ ROSA A.,
PÉREZMARTÍNEZ CLAUDIA,
ÁLVAREZ JAVIER ESPINOSA,
NAVARRETE ALEX J. DURÁN,
GARCÍAIGLESIAS MARIA J.
Publication year - 2006
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/j.1365-3164.2005.00499.x
Subject(s) - retinoic acid , in utero , epidermis (zoology) , fetus , keratin , morphogenesis , biology , tretinoin , gestation , andrology , retinoid , endocrinology , medicine , pregnancy , cell culture , anatomy , genetics , gene
Epidermal morphogenesis was studied in vivo following prenatal exposure to retinoic acid (RA). In pregnant mice, a single oral dose of RA on day 11.5 of gestation failed to induce histological changes in fetal epidermal development except in epidermal thickness. Epidermal thickness increased from 16.5 days post‐coitum (dpc) onwards, and temporal and spatial epidermal modifications in keratins K5 and K14 related to proliferative activity of keratinocytes were observed. An RA effect on cell proliferation was supported by a statistically significant increase in the number of epidermal S‐phase cells, containing BrdU–incorporated DNA in RA‐exposed mice compared with nonexposed animals. The prolonged in utero action of RA on epidermal proliferative activity in fetuses and newborns suggests a long‐term RA effect that may play a role on the development and evolution of diseases in adult skin.