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Immunolocalization of aquaporin‐5 expression in sweat gland cells from normal and anhidrotic horses
Author(s) -
BOVELL DOUGLAS L.,
LINDSAY SUSAN L.,
CORBETT ALISTAIR D.,
STEEL CATHERINE
Publication year - 2006
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/j.1365-3164.2005.00498.x
Subject(s) - sweat gland , sweat , aquaporin 1 , anatomy , biology , medicine , andrology , endocrinology , water channel , engineering , mechanical engineering , inlet
Western blot analysis showed that sweat gland cells from freely sweating horses expressed the water channel aquaporin‐5 (AQP‐5). Immunohistochemistry revealed a strong AQP‐5‐like activity reaction at the apical membrane of the glandular secretory cells, which was absent from the surrounding myoepithelium and all other skin structures. In anhidrotic horses, AQP‐5 was also found at the apical membrane of the luminal sweat gland cells, but the level of expression reduced with the length of time that the animal had displayed anhidrosis. The level of AQP‐5 expression was substantially reduced in animals with long‐term anhidrosis, hence implicating water channel impairment as a possible factor in the development of this disorder.