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Mast cell morphometry of cutaneous wounds treated with an autacoid gel: a placebo‐ controlled study
Author(s) -
Abramo F.,
Salluzzi D.,
Leotta R.,
Noli C.,
Auxilia S.,
Mantis P.,
Lloyd D.
Publication year - 2004
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/j.1365-3164.2004.411_59.x
Subject(s) - medicine , granulation tissue , autacoid , dermis , wound healing , mast cell , bolus (digestion) , surgery , pathology , receptor , immunology
Mast cells (MC) are pivotal in wound healing. Adelmidrol, a synthetic autacoid, can down‐regulate MC degranulation and potentially accelerate healing and improve cosmetic outcome. This study evaluated an Adelmidrol gel on MC in experimental wounds. Two rows of six 5‐mm wounds were created in 10 beagles and allowed to heal; one row was treated daily with therapeutic gel and the other with vehicle only. Treated and control 8‐mm samples of wounds were taken after 1, 2, 4, 8 and 14 days, fixed in formalin and paraffin embedded. Four‐micrometer sections were stained with toluidine blue for MC counts and densitometry. Counts (MC/mm 2 ) were obtained from areas lateral to and within the ulcer/granulation tissue, and in deep dermis/panniculus. A decreasing trend in MC counts occurred throughout days 1 (3.8 ± 11.3), 2 (4.3 ± 14.9) and 4 (4.3 ± 9.9) compared to day 0 (5.0 ± 10.6), followed by an increase at day 8 (6.0 ± 12.3) and a significant increase at day 14 (10.0 ± 18) compared to days 1, 2 and 4 in granulation tissue and perifollicular areas ( P < 0.05) and deep dermis/panniculus ( P < 0.01). No difference in MC counts was detected between treated andcontrol wounds. Preliminary data from two dogs showed granulation density was higher in treated wounds from day 2 (119.1 ± 27.4 vs . 69.4 ± 14.2) until day 14 (92.4 ± 22.1 vs. 67.9 ± 12.9). In conclusion, MC populations seem to change during wound healing. The autacoid gel did not alter MC numbers, but decreased degranulation. Funding: Royal Veterinary College, Innovet.