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FC‐58
A new method for measuring canine transepidermal water loss
Author(s) -
Yoshihara T.,
Endo K.,
Konno K.,
Iwasaki T.
Publication year - 2004
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/j.1365-3164.2004.411_58.x
Subject(s) - transepidermal water loss , skin barrier , coat , medicine , zoology , materials science , dermatology , pathology , biology , composite material , stratum corneum
Many studies have focused on the relationship between barrier damage and human atopic dermatitis. Currently, evaluating human skin barrier function depends on measuring transepidermal water loss (TEWL) by Evaporimeter. However, applying this method clinically to dogs is impractical due to the influences of air turbulence and vapour from the hair coat. To minimize these influences, we developed and evaluated a new method for measuring TEWL using a closed chamber system. Dry air was injected into a closed chamber placed on the dog's skin surface, and the vapour content of recovered air was detected with a quartz crystal sensor as a frequency variance. At first, a frequency decrease attributable to hair coat vapour was observed, and then the frequency gradually increased and became constant. The steady state value was used to calculate TEWL. It was unnecessary to restrict a dog's movement to control independent environmental effects. Transepidermal water loss was measured in 18 beagles with normal skin at seven different anatomical sites. Results confirmed it was possible to measure TEWL without clipping hair at the inguinal site. Funding: Kao Corporation.