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FC‐52
Cross‐reactivity between house dust, sarcoptic and storage mites in dogs with atopic dermatitis
Author(s) -
Virchow F.,
Bigler B.
Publication year - 2004
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/j.1365-3164.2004.411_52.x
Subject(s) - allergen , immunoglobulin e , mite , sarcoptes scabiei , serology , acari , antibody , house dust mite , immunology , cross reactivity , dust mites , chemistry , biology , microbiology and biotechnology , cross reactions , allergy , ecology
In this study, sera from 43 dogs that previously tested positive to different mites with IgE antibodies were used for immunoblotting to screen for common allergen bands. The use of gradient gels allowed the search for small (<20 kD) and large (up to 250 kD) proteins. Anticanine‐IgE detection antibody (D9) was used for immunostaining. The mites showed species‐specific allergen patterns; yet, we had reason to believe that several proteins might be conserved among mites and cause cross‐reactivity. Often observed bands for Acarus siro had sizes of approximately 43, 45, 54, 63, 71 and 96 kD; for Dermatophagoides farinae , 55, 68, 96, 98, 104 and 114 kD; for Dermatophagoides pteronyssinus , 98 kD; for Lepidoglyphus destructor , 45, 46, 55 and 107 kD; for Sarcoptes scabiei , 98 and 104 kD; and finally for Tyrophagus puterescientiae , 55, 66, 68, 70, 89 and 98 kD. We have shown that cross‐reactivity can lead to false‐positive results if serological tests or intradermal tests are carried out with total mite protein extracts. Funding: Laupeneck AG.