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Immunohistochemical detection of c‐kit in canine cutaneous mast cell tumours
Author(s) -
Cadiergues M. C.,
DegorceRubiales F.,
Abadie J.,
Amardeilh M.,
Delverdier M.
Publication year - 2004
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/j.1365-3164.2004.411_15.x
Subject(s) - labelling , immunohistochemistry , mast cell , homogeneous , pathology , cytoplasm , grading (engineering) , nucleus , staining , cell , microbiology and biotechnology , biology , medicine , immunology , ecology , biochemistry , physics , genetics , thermodynamics
The objectives of this study were to evaluate the immunohistochemical expression of c‐kit by mast cells in canine mast cell tumours (MCT) and to correlate this expression with the tumour grade. Cutaneous MCT from 60 dogs were graded histologically (Patnaik grading system), and immunohistochemical staining for c‐kit was performed. Evaluation criteria were the percentage of labelled cells, the homogeneous/mixed character, the intensity (0–3) and the location of labelling, either cell membrane or cytoplasmic (diffuse, granular, or near the nucleus). For grade I tumours (20 dogs), c‐kit expression was detected in 100%: 80% showed homogeneous labelling, and 90% contained 80–100% labelled mast cells. Labelling was detected on the cell membrane (65%) and near the nucleus (75%). For grade II tumours (20 dogs), c‐kit expression was detected in 100%: 61% showed homogeneous labelling and 96% contained 80–100% labelled mast cells. Labelling was detected on the cell membrane (74%) and close to the nucleus (74%). For grade III tumors (20 dogs), c‐kit expression was detected in 100%: 71% showed homogeneous labelling, 21% contained 10–50% labelled mast cells, 21% contained 50–80% labelled cells, and 54% contained 80–100% labelled cells. Most of the labelled cells had a poorly labelled membrane (75%), with labelling near the nucleus being undetectable or low in 50% of the tumours. Other cytoplasmic labelling was detected in 21% of the tumours (10% of grade I and 13% of grade II). Results suggest that c‐kit can be used as a reliable marker for canine MCT, notably for undifferentiated MCT. Funding: National Veterinary School of Toulouse.