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Immunity to bovine trichophytosis
Author(s) -
Faldyna M.,
Krejčí J.,
Obořilová E.,
Rybnikář A.
Publication year - 2004
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/j.1365-3164.2004.411_03.x
Subject(s) - antigen , immunology , immune system , antibody , immunity , population , biology , medicine , virology , environmental health
Bovine trichophytosis is a mycotic cutaneous disease caused mainly by the dermatophyte Trichophyton verrucosum . Cell‐mediated immunity (CMI) is reported to play a crucial role in defense against the disease. The objective of this study was to investigate the immune response developed after administration of a vaccine containing a live immunogenic culture of T. verrucosum . For the detection of CMI, we used an antigen‐specific lymphocyte proliferation test measured by both 3 H‐thymidine incorporation and flow cytometry, and specific antigen‐induced production of IFNγ. We also tested specific antibody production by an indirect ELISA test. After vaccination by either the subcutaneous, intramuscular, or intradermal route, animals developed solid in vitro CMI 1 month after revaccination. CD4 + lymphocytes were the basic cell population activated by Trichophyton antigen in vitro . IgM and IgG antibody responses were detected from postvaccination days 3 and 7, respectively. Animals expressing higher levels of CMI displayed higher ratios of IgG2:IgG1 (a Th1 pattern of immune response). Using the challenge test, protection was higher after intramuscular > subcutaneous >intradermal routes of administration. We conclude that administration of a vaccine containing a live immunogenic culture of T. verrucosum leads to the development of protective immunity against bovine trichophytosis, and that the results of our laboratory tests correlated well with the results of challenge tests. Funding: Ministry of Agriculture of the Czech Republic.