Premium
Diversidad genética y multiplicidad de genotipos en las infecciones por Plasmodium falciparum de individuos sintomáticos en la región marítima de Togo
Author(s) -
Awaga Kwami L.,
Missihoun Tag D.,
Karou Simplice D.,
Djadou Koffi E.,
Chabi Nicodème W.,
Akati Afoua,
LawsonHukportie Afi,
Amevo Kokou,
Djegno Kodjovi,
Guéant Jean L.,
Sanni Ambaliou,
Amouzou Sabiba K. E.
Publication year - 2012
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/j.1365-3156.2011.02913.x
Subject(s) - genotyping , genotype , biology , malaria , allele , plasmodium falciparum , immunology , genetic diversity , population , virology , genetics , medicine , gene , environmental health
Summary Objective To assess the genotype prevalence and the multiplicity of Plasmodium falciparum infections in the maritime region of Togo. Methods We enrolled 309 symptomatic individuals aged from 6 months to 15 years from Bè/Lomé and Tsévié, two malaria endemic zones. The number and the proportions of merozoite surface proteins 1, 2 and 3 genotypes in patients were determined using capillary electrophoresis genotyping. We further investigated the possible association between transaminases and homocysteine, and the severity of the disease. Results Of the 309 samples genotyped, 210 tested positive to msp‐1, 227 to msp‐2 and 193 to msp‐3. The nested PCR revealed 22 different alleles for the allelic family msp‐1, 33 for msp‐2 and 13 for msp‐3. At each locus, the family distribution was 54.58% of K1, 25% of MAD20 and 20.42% of RO33 for msp‐1, and 51.71% and 48.29% of FC27 and 3D7, respectively, for msp‐2. For all these allelic variants, the distribution was associated with neither the severity of malaria nor the zone of habitation. Pearson correlation coefficients between either the levels of homocysteine or the transaminase and the severity of the disease were very low. Conclusion The severity of malaria was not associated with higher multiplicity of infections and did not appear restricted to particular genotypes. More comprehensive explorations including immunity, genetic factors, nutritional and sociologic status of the population could clarify the situation.