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Genotipaje de los marcadores genéticos polimórficos MSP3α y MSP3β de aislados de campo de Plasmodium vivax provenientes de áreas endémicas de malaria de Tailandia
Author(s) -
Rungsihirunrat Kanchana,
Chaijaroenkul Wanna,
Siripoon Napapoon,
Seugorn Aree,
NaBangchang Kesara
Publication year - 2011
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/j.1365-3156.2011.02771.x
Subject(s) - genotyping , biology , restriction fragment length polymorphism , plasmodium vivax , polymerase chain reaction , genetics , gene , genetic marker , allele , polymorphism (computer science) , population , genotype , malaria , plasmodium falciparum , immunology , demography , sociology
Summary Two polymorphic marker genes, merozoite surface protein 3α ( PvMSP3α ) and merozoite surface protein 3β ( PvMSP3β ), from 100 Plasmodium vivax field isolates, were investigated using polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP). Genotyping of PvMSP3α and PvMSP3β revealed marked polymorphisms in length and sequence. Three major types of PvMSP3α (Type A, B and C) and two major types of PvMSP3β (Type A and B) were detected based on the length of PCR products. Fourteen alleles of both genes with difference frequencies were distinguished by restriction fragment length polymorphism, and these results strongly support that P. vivax isolates in Thailand are markedly diverse. PvMSP3α and PvMSP3β are reliable polymorphic markers for population genetic analysis of P. vivax , and PCR/RFLP provides a powerful method for genotyping and identification of mixed parasite infections without requirement of gene sequencing.