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Variabilidad genética en el gen de la aldolasa de P.vivax en aislados Coreanos y la sensibilidad de la prueba para malaria Binax Now
Author(s) -
Cho ChiHyun,
Nam Myoung Hyun,
Kim Jang Su,
Han Eun Taek,
Lee Won Ja,
Oh Jun Seo,
An Seong Soo A.,
Lim Chae Seung
Publication year - 2011
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/j.1365-3156.2010.02691.x
Subject(s) - aldolase a , plasmodium vivax , biology , fructose bisphosphate aldolase , malaria , virology , genetics , aldolase b , immunology , plasmodium falciparum , biochemistry , enzyme
Summary Introduction of rapid malaria diagnostic tests (RDT) initiated numerous field evaluations in various epidemiologic settings. But the efficiency of some RTD kits based on aldolase raised reservations for direct implementation of RDT into clinical settings. We performed Binax Now malaria test in 84 Korean Plasmodium vivax isolates and compared it with the traditional Giemsa stain microscopy test as the reference standard. The sensitivity of Binax Now was 62.0% for P. vivax cases (52/84, 95% CI 51.2–71.6%) with 100.0% specificity (50/50, 95% confidence interval 92.9–100%). After the aldolase gene sequence analysis of 84 isolates, two synonymous mutations in aldolase gene were identified in both Binax Now positive and negative samples. No significant association between the mutations and Binax Now malaria tests was found. Thus, the genetic variability would not explain the poor performance of P. vivax RDTs by detecting aldolase in ROK isolates.