Premium
Alta susceptibilidad de Aedes aegypti al virus Chikungunya en las Indias Francesas Occidentales y la Guyana Francesa
Author(s) -
Girod Romain,
Gaborit Pascal,
Marrama Laurence,
Etienne Manuel,
Ramdini Cédric,
Rakotoarivony Ignace,
Dollin Christelle,
Carinci Romuald,
Issaly Jean,
Dusfour Isabelle,
Gustave Joël,
YpTcha MarieMichelle,
Yébakima Andre,
Failloux AnnaBella,
Vazeille Marie
Publication year - 2011
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/j.1365-3156.2010.02613.x
Subject(s) - west indies , chikungunya , aedes aegypti , geography , dengue fever , yellow fever , aedes , virology , biology , virus , ethnology , history , ecology , larva
Summary Objectives To estimate the vector competence of Aedes aegypti populations sampled from distinct anthropogenic environments in French Guiana, Guadeloupe and Martinique for the strain CHIKV 06.21. Methods F 1 /F 2 females were orally infected at titres of 10 6 and 10 7.5 pfu/ml in blood‐meals. Disseminated infection rates (DIR) of mosquitoes were estimated using indirect fluorescent antibody assay on heads’ squashes, 7 or 14 days post‐infection (pi). Results At a titre of 10 7.5 pfu/ml, DIR ranged from 88.9% to 100.0% and were not significantly different whether assessed at day 7 or 14 pi. At a titre of 10 6 pfu/ml, DIR observed 7 days pi ranged from 37.6 to 62.0%. Conclusions Ae. aegypti from French Guiana and French West Indies are highly competent to transmit CHIKV. An evaluation of DIR 7 days rather than 14 days pi is adequate to estimate vector competence. The titre of 10 6 pfu/ml allows us to distinguish Ae. aegypti populations originating from distinct environments (dense or diffuse housing) by their vector competence. This assessment is a prerequisite to better evaluate the potential risk of Chikungunya outbreaks once the virus is introduced from endemic regions.