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Sensitivity and specificity of the Leishmania OligoC‐TesT and NASBA‐oligochromatography for diagnosis of visceral leishmaniasis in Kenya
Author(s) -
Basiye Frank L.,
Mbuchi Margaret,
Magiri Charles,
Kirigi George,
Deborggraeve Stijn,
Schoone Gerard J.,
Saad Alfarazdeg A.,
ElSafi Sayda,
Matovu Enock,
Wasunna Monique K.
Publication year - 2010
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/j.1365-3156.2010.02548.x
Subject(s) - visceral leishmaniasis , nasba , leishmaniasis , leishmania , medicine , confidence interval , diagnostic test , immunology , gastroenterology , virology , biology , veterinary medicine , parasite hosting , nucleic acid sequence , genetics , dna , world wide web , computer science
Summary Objective To estimate the sensitivity and specificity of the OligoC‐TesT and nucleic acid sequence‐based amplification coupled to oligochromatography (NASBA‐OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya. Methods Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays. Results The Leishmania OligoC‐TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90–98.8%) and a specificity of 88.8% (95% CI: 81–93.6%), while the sensitivity and specificity of the NASBA‐OC were 79.8% (95% CI: 67–87%) and 100% (95% CI: 96.3–100%), respectively. Conclusion Our findings indicate high sensitivity of the Leishmania OligoC‐TesT on blood while the NASBA‐OC is a better marker for active disease.