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Susceptibilidad in vitro de aislados de Leishmania (Viannia) braziliensis y Leishmania (Leishmania) amazonensis Brazilian frente al antimoniato de meglumina y la anfotericina B
Author(s) -
ZauliNascimento Rogéria C.,
Miguel Danilo C.,
YokoyamaYasunaka Jenicer K. U.,
Pereira Ledice I. A.,
Pelli de Oliveira Milton A.,
RibeiroDias Fátima,
Dorta Miriam L.,
Uliana Silvia R. B.
Publication year - 2010
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/j.1365-3156.2009.02414.x
Subject(s) - meglumine antimoniate , leishmania , leishmaniasis , leishmania braziliensis , microbiology and biotechnology , amphotericin b , kinetoplastida , biology , virology , amastigote , protozoa , protozoal disease , antifungal , cutaneous leishmaniasis , parasite hosting , immunology , malaria , world wide web , computer science
Summary Resistance of Leishmania parasites to specific chemotherapy has become a well‐documented problem in the Indian subcontinent in recent years but only a few studies have focused on the susceptibility of American Leishmania isolates. Our susceptibility assays to meglumine antimoniate were performed against intracellular amastigotes after standardizing an in vitro model of macrophage infection appropriate for Leishmania (Viannia) braziliensis isolates. For the determination of promastigote susceptibility to amphotericin B, we developed a simplified MTT‐test. The sensitivity in vitro to meglumine antimoniate and amphotericin B of 13 isolates obtained from Brazilian patients was determined. L. (V.) braziliensis isolates were more susceptible to meglumine antimoniate than Leishmania (Leishmania) amazonensis . EC 50 , EC 90 and activity indexes (calculated over the sensitivity of reference strains), suggested that all isolates tested were susceptible in vitro to meglumine antimoniate, and did not show association with the clinical outcomes. Isolates were also uniformly susceptible in vitro to amphotericin B.