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Diagnóstico por PCR de la leishmaniasis visceral en una región endémica: distrito de Mymensingh, Bangladesh
Author(s) -
Alam Mohammad Zahangir,
Shamsuzzaman Abul Khair M.,
Kuhls Katrin,
Schönian Gabriele
Publication year - 2009
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/j.1365-3156.2009.02254.x
Subject(s) - visceral leishmaniasis , leishmaniasis , endemic diseases , geography , medicine , environmental protection , socioeconomics , environmental health , immunology , sociology
Summary Detection of Leishmania parasites in a clinical sample is necessary to confirm a suspected case of leishmaniasis. We compared the sensitivity of internal transcribed spacer 1‐PCR (ITS 1‐PCR) assay for parasite diagnosis with that of microscopic detection in clinical samples from kala‐azar (KA) or post‐kala‐azar dermal leishmaniasis (PKDL) suspects in Mymensingh. Of 39 specimens collected from 35 KA and four PKDL suspects, 26 were positive by microscopic examination of smears from bone marrow and skin exudates; 38 specimens spotted on filter paper and 27 of the 28 Giemsa‐stained slides tested by PCR proved positive by ITS1‐PCR.