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Evaluación del método de Kudoh para el cultivo de Mycobacterium tuberculosis en áreas rurales
Author(s) -
Jaspe Rossana C.,
Rojas Yeimy M.,
Flores Lalis A.,
Sofia Toro E.,
Takiff Howard,
De Waard Jacobus H.
Publication year - 2009
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/j.1365-3156.2009.02236.x
Subject(s) - sputum , medicine , mycobacterium tuberculosis , tuberculosis , microbiology and biotechnology , biology , pathology
Summary Objective To compare the simple, swab ‘Kudoh method’ for culturing Myobacterium tuberculosis from sputum samples, to the standard Petroff digestion‐decontamination procedure. The Kudoh method, which requires no centrifugation and takes only 4–5 min per sample, was also evaluated for its performance in a rural setting. Methods Two hospital laboratories in Caracas, Venezuela processed 314 sputum samples, in parallel, with both methods. Separately, sputum specimens were cultured with the Kudoh swab method in a field environment with minimal laboratory facilities. Results In the hospital laboratories, the sensitivity of the Kudoh swab method was comparable to that of the standard Petroff culture procedure. The swab method also performed satisfactorily in the field, improving the diagnostic sensitivity by 21% over microscopic examination alone. Conclusion The Kudoh swab method is an acceptable alternative for culturing mycobacteria that is particularly suitable for rural laboratories lacking adequate infrastructure for the Petroff method.