Premium
Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for the serodiagnosis of neurocysticercosis
Author(s) -
Arruda G. C.,
Da Silva A. D. T.,
Quagliato E. M. A. B.,
Maretti M. A.,
Rossi C. L.
Publication year - 2005
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/j.1365-3156.2005.01480.x
Subject(s) - taenia crassiceps , neurocysticercosis , taenia solium , cysticercosis , antigen , parasite hosting , helminthiasis , taenia , biology , virology , immunology , medicine , pathology , cestoda , helminths , computer science , world wide web
Summary We evaluated the usefulness of seven cysticercal antigen extracts, four from Taenia solium cysticerci (whole parasite‐TsoW, membrane‐TsoMe, vesicular fluid‐TsoVF and scolex‐TsoSc) and three from T. crassiceps cysticerci (whole parasite‐TcraW, membrane‐TcraMe and vesicular fluid‐TcraVF), for serodiagnosis of neurocysticercosis with an enzyme‐linked immunosorbent assay (ELISA). Cysticercus ‐specific IgG were screened in serum samples from 23 patients with neurocysticercosis, 32 patients with other infections and 48 healthy persons. The best results were obtained with the TsoVF‐ELISA (91.3% sensitivity; 96.2% specificity) and TcraVF‐ELISA (91.3% sensitivity; 95% specificity). The ELISA done with whole parasite and membrane extracts from cysts of T. solium and T. crassiceps and the scolex extract from T. solium cysts showed a low performance in terms of sensitivity, ranging from 47.8% to 73.9%. None of the antigen preparations from T. solium and T. crassiceps cysticerci used in this study showed outstanding performance for the serodiagnosis of neurocysticercosis. However, considering the results obtained with the seven antigen preparations, vesicular fluid from T. solium and T. crassiceps cysticerci may be useful for detecting specific antibodies in sera from patients with neurocysticercosis.