Premium
Short Communication: Palestinian infantile visceral leishmaniasis caused by a genetic variant of Leishmania infantum belonging to a new zymodeme
Author(s) -
Bader Khaldoun A.,
Schnur Lionel F.,
Nasereddin Abedelmajeed,
Pratlong Francine,
Dedet JeanPierre,
Shaheen Loay,
Yousef Obaida,
Greenblatt Charles L.
Publication year - 2005
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/j.1365-3156.2005.01419.x
Subject(s) - leishmania infantum , visceral leishmaniasis , leishmania , leishmaniasis , restriction enzyme , biology , restriction fragment length polymorphism , polymerase chain reaction , malate dehydrogenase , virology , endonuclease , genetics , parasite hosting , gene , enzyme , biochemistry , world wide web , computer science
Summary The parasites causing a Palestinian case of infantile visceral leishmaniasis (IVL) and those from four dogs from the Jenin District were identified serologically, biochemically and molecular biologically as Leishmania infantum , showing dogs act as a reservoir. The strain from the human case was distinct because of its unique 200‐bp kDNA‐polymerase chain reaction (PCR) component in its restriction fragment length polymorphism (RFLP) profile after digestion with the endonuclease Rsa I, and by the electrophoretic mobility of its malate dehydrogenase (MDH 140 ), designating it the reference strain of a new zymodeme of L. infantum , MON‐281.