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Short Communication: Detection of dengue virus antigens in desiccated mosquitoes: an improved tool for surveillance
Author(s) -
Thenmozhi V.,
Kabilan L.,
Philip Samuel P.,
Dash A. P.
Publication year - 2005
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1111/j.1365-3156.2004.01360.x
Subject(s) - dengue fever , dengue virus , aedes aegypti , virology , immunoassay , antigen , biology , aedes , flavivirus , virus , veterinary medicine , medicine , immunology , antibody , botany , larva
Summary We developed a surveillance tool to monitor dengue virus (DENV) infection in vector mosquitoes using a dengue antigen‐capture enzyme immunoassay (EIA) on desiccated specimens stored at room temperature (RT). We tested the effect of storage on the stability of DEN1, DEN2, DEN3 and DEN4 antigens. Although desiccated infected Aedes aegypti mosquitoes stored between 31 and 34 °C (RT) showed greater reactivity in the EIA than those stored at −80 °C, a significant difference between the two was observed only with DEN2. Storage between 31 and 34 °C for up to 4 weeks (the longest period tested) did not affect the reactivity in the EIA, indicating the stability of DENV antigens.