Premium
Red cell concentrate storage and transport temperature
Author(s) -
Hancock V.,
Cardigan R.,
Thomas S.
Publication year - 2011
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.1365-3148.2011.01083.x
Subject(s) - blood preservation , chemistry , medicine , andrology
Background and Objectives: This study investigated the current UK guidelines for storage and transport of red cell concentrates (RCC) in saline, adenine, glucose and mannitol (SAGM). The guidelines stipulate storage at 2–6 °C but allow exposure to between 1–10 °C core temperature in a single occurrence of less than 5 h and a surface temperature of 2–10 °C for no more than 12 h during transportation. Methods and Materials: Twenty RCC units in SAGM were selected on the day of blood collection (day 0) and in vitro quality was tested pre‐ and post‐temperature deviation at 10 °C and up to day 42 of storage. Each group of 10 RCC units was incubated for either 12 h or for both 5 and 12 h. Results: Haemolysis was below the 0·8% UK limit at day 42 in all units, although there was an unexpected trend towards lower haemolysis in packs incubated for 5 and 12 h rather than just 12 h alone. Supernatant potassium was significantly higher than reference data on day 35 ( P < 0·05) with a maximum of 58 mmol L −1 and day 42 ( P < 0·001). All units incubated at 10 °C had comparable levels of adenosine triphosphate and, 2,3‐diphosphoglycerate to reference data from previous studies, throughout storage. Conclusion: These results suggest that exposure to 10 °C for 12 h or for 5 and 12 h did not adversely affect in vitro red cell quality for the remainder of the components shelf life.