Premium
Serial oxygen equilibrium and kinetic measurements during RBC storage
Author(s) -
Gelderman M. P.,
Yazer M. H.,
Jia Y.,
Wood F.,
Alayash A. I.,
Vostal J. G.
Publication year - 2010
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.1365-3148.2010.01016.x
Subject(s) - oxygen , chemistry , biophysics , blood preservation , kinetics , biochemistry , biology , physiology , physics , organic chemistry , quantum mechanics
Objectives: To contribute to the understanding of the biochemical changes associated with the RBC storage lesion. Aim: To investigate changes in O 2 equilibrium and on/off kinetic rates during routine cold storage. Background: As RBCs are stored between 1 and 6°C numerous biochemical changes occur within the RBCs, including changes in the properties of the haemoglobin itself. This study serially analysed for the first time the O 2 equilibrium and on/off kinetic rates across the RBC membrane during routine storage. Methods/Materials: The oxygen binding ( k on ) and offloading ( k off ) constants were measured in fresh RBCs and then in AS‐5‐preserved RBCs at weekly intervals, along with oxygen equilibrium curves (OECs), 2,3‐Diphosphoglycerate (2,3‐DPG), p50 and the Hill number ( n ). Results: The k on increased slightly as the 2,3‐DPG and p50 decreased during storage, whereas the k off remained largely unchanged. The OECs demonstrated the expected increase in O 2 affinity, whereas the Hill number was unchanged during storage. Conclusion: In spite of the biochemical, structural and functional changes associated with the storage of RBCs, their in vitro interactions with oxygen were largely preserved through 42 days of storage.