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The Quantitation of D Antigenic Sites in Partial D and Weak D Variants by Flowcytometry
Author(s) -
Kulkarni Swati,
Mohanty Dipika,
Gupte Snehalata,
Vasantha K,
Joshi Sanmukh
Publication year - 2005
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.1365-3148.2005.00554ak.x
Subject(s) - antigen , epitope , serology , microbiology and biotechnology , monoclonal antibody , chemistry , population , phenotype , antibody , biology , genetics , gene , biochemistry , medicine , environmental health
Aim The aim of the study is to quantitate D antigenic sites on RBC in weak D and partial D variants in Indian population by flowcytometric method. Method Dantigenic sites were estimated in 48 cases of partial D, eight cases of weak D (identified by serological methods) and normal Rh phenotypes subjects by flowcytometric technique. The indirect immunoflourescence method was employed using thirty epitope specific monoclonal anti‐D as primary antibody. The D antigenic sites were calculated using standard RBC with known D antigenic sites. Result The mean (±I S.D) D antigenic sites per RBC in weak D and partial D were 4503 ± 2067 and 6750 ± 3426 respectively. In R1R1 phenotype the range of D antigenic sites was 16500 ± 2500 and in R1r it was 10600 ± 2520. In four cases of weak D sites were in range of 1700 to 3500 and in other four it was 5000 to 7200 D sites/RBC. Among partial D variants DVI had minimum, and DVa had maximum number of D sites. Conclusion Flowcytometry is a very good tool for demonstrating minor differences in D antigen sites in partial D and weak D when serological methods are not conclusive.