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Anti‐D quantification by flow cytometry: an alternative to the Auto Analyser?
Author(s) -
Austin E. B.,
McIntosh Y.,
Hodson C.,
Lee D.
Publication year - 1995
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.1365-3148.1995.tb00229.x
Subject(s) - flow cytometry , standard curve , analyser , chromatography , reagent , chemistry , fluorescence , cytometry , microbiology and biotechnology , biology , physics , quantum mechanics
SUMMARY. A flow cytometry method for quantifying levels of anti‐D is described. The method is based on the indirect antiglobulin test and uses an FITC Fab anti‐human IgG reagent for detection of bound anti‐D. A standard curve is generated from the fluorescence measured by flow cytometry using the British Standard anti‐D preparation 73/515. The fluorescence obtained from test samples by flow cytometry is converted into IU/mL anti‐D, using the standard curve. In a limited study with 42 serum samples the assay showed good correlation with results obtained using the AutoAnalyser for sera with anti‐D levels less than 100 IU/mL. The method is simple to perform and has potential as a replacement for the AutoAnalyser.