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Monoclonal antibodies to high‐incidence Kell epitopes: characterization and application in automated screening of donor samples
Author(s) -
Inglis G.,
Fraser R. H.,
McTaggart S.,
Sheridan R.,
Forrest A. H.,
Mitchell R.
Publication year - 1994
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.1365-3148.1994.tb00273.x
Subject(s) - epitope , monoclonal antibody , antibody , phenotype , antigen , microbiology and biotechnology , monoclonal , immunology , biology , genetics , gene
SUMMARY. Monoclonal antibodies, LM313/706 and LM357/828, recognize high‐frequency epitopes which are absent on red cells of Ko phenotype. Both antibodies have proved suitable for automated screening of blood donor samples, with 5 ml of each culture supernatant sufficient to screen 3,000 donor samples. In a screen of 45,545 samples, LM313/706 revealed 11 samples (1: 3686) of Kp(a + b‐) phenotype. Thirty‐seven samples of K + k‐ phenotype were identified in 15,235 samples screened with LM357/828 antibody (0.24%). The epitope recognized by LM313/706 was inactivated by 2mm DTT suggesting a possible association with the Js a /Js b antigen structure. The expression of the k‐like epitope detected by LM357/828 antibody was found to be influenced by pH change.