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A simplified method for large‐scale HPA‐la phenotyping for antenatal screening
Author(s) -
Metcalfe P.,
Doughty H. A.,
Murphy M. F.,
Waters A. H.
Publication year - 1994
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.1365-3148.1994.tb00239.x
Subject(s) - medicine , typing , antenatal screening , screening test , neonatal alloimmune thrombocytopenia , scale (ratio) , immunology , pregnancy , biology , pediatrics , fetus , genetics , physics , quantum mechanics
SUMMARY. A simplified method for large‐scale HPA‐la phenotyping of platelets was developed for use in an antenatal screening programme for fetomaternal alloimmune thrombocytopenia (FMAIT). The test was based on the MAIPA assay, which was modified for antigen‐typing with a well‐characterized anti HPA‐la reagent. The resulting assay gave reliable results, was inexpensive and allowed testing of large batches using semiautomated equipment.