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Human platelet antigen‐1 (Zw) typing of fetuses by analysis of polymerase chain reaction‐amplified genomic DNA from amniocytes
Author(s) -
Simsek S.,
Christiaens G. C. L. M.,
Kanhai H. H. H.,
Beekhuis J. R.,
Bleeker P. M. M.,
Vlekke A. B. J.,
Goldschmeding R.,
Borne A. E. G.
Publication year - 1994
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.1365-3148.1994.tb00238.x
Subject(s) - polymerase chain reaction , typing , neonatal alloimmune thrombocytopenia , genomic dna , prenatal diagnosis , amniocentesis , fetus , genotype , microbiology and biotechnology , allele , antigen , biology , restriction enzyme , medicine , genetics , dna , pregnancy , gene
SUMMARY. Prenatal typing for the human platelet antigens‐1 (HPA) permits identification of a fetus at risk for neonatal alloimmune thrombocytopenia (NAITP) in cases of HPA‐1 incompatibility in which the father is heterozygous for the HPA‐la antigen. Diagnostic cordocentesis and phenotyping of the fetal platelets are used for this purpose. We applied allele‐specific restriction enzyme analysis on polymerase chain reaction (PCR)‐amplified DNA purified from amniocytes. This assay allows early second trimester typing for HPA‐1 alleles. We were able to determine the genotype of three fetuses at risk. Iatrogenic fetal loss is lower with amniocentesis than with cordocentesis. Therefore, this technique is a welcome addition to the antenatal management of NAITP.