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Immunochemical characterization of the anti‐B response in an ABO‐incompatible blood transfusion: presence of antibodies recognizing glucosylceramide
Author(s) -
Rydberg L.
Publication year - 1993
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.1365-3148.1993.tb00113.x
Subject(s) - abo blood group system , antibody , radioimmunoassay , immunology , blood type (non human) , medicine , rh blood group system , blood transfusion
Summary. A male patient of blood group A was accidentally given two units of group B blood when operated upon because of injury. This resulted in an immediate haemolytic transfusion reaction. The patient was treated with exchange transfusions in order to remove the incompatible red cells and thus prevent renal damage. Two weeks after the transfusion, an anti‐B humoral immune response appeared. The antibodies were mainly IgG and IgA types, of IgG3 and IgA1 subclasses. The anti‐B antibodies seemed specific for the B trisaccharide [Galα1–3(Fucα1–2)Gal] as tested by radioimmunoassay and chromatogram binding assay. No antibodies recognizing part of the core saccharide chain could be detected, i.e. antibodies capable of differentiating between B type 1 and B type 2 chains did not occur. An unsuspected finding was that the patient had IgG antibodies recognizing glucosylceramide (and weakly also galactosylceramide) before and after the transfusion. These antibodies were still present 9 years after the ABO‐incompatible blood transfusion.