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Platelet counting using plasma platelet concentrate samples
Author(s) -
Lord R. A.,
Smith G. A.,
Nightingale M. J.,
Boulton F. E.
Publication year - 1992
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.1365-3148.1992.tb00156.x
Subject(s) - coulter counter , platelet , mean platelet volume , dilution , chromatography , medicine , mathematics , chemistry , physics , biology , microbiology and biotechnology , thermodynamics
SUMMARY. Platelet counting using samples of plasma from platelet concentrates prepared for transfusion was assessed. The methods employed included a manual phase‐contrast method, and counting with Coulter S Plus and Sysmex E‐2500 counters. All methods were reproducible (mean CV of 4.9, 2.2 and 1.4%, respectively). However, neat samples of platelet concentrates analysed by Coulter counter were inaccurate (mean count of 863.8 times 10 9 /1 compared to 1018.9 times 10 9 /1 counted manually). Moreover, the Coulter platelet counts were non‐linear above 900 times 10 9 /1, whereas the E‐2500 platelet counts were linear to 2700 times 10 9 /1. A one‐in‐three pre‐dilution was required to obtain accurate, linear counts with the Coulter counter, whereas the E‐2500 was accurate without pre‐dilution (mean count of 1030.2 times 10 9 /1 compared to 1018.9 times 10 9 /1 counted manually). In conclusion, the method of platelet counting may affect true platelet yields.