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B v : a distinct category of B sub‐group among Chinese blood donors in Hong Kong
Author(s) -
Mak K. H.,
Voak D.,
Chu R. W.,
Leong S.,
Chua K. M.
Publication year - 1992
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.1365-3148.1992.tb00146.x
Subject(s) - polyclonal antibodies , monoclonal antibody , epitope , monoclonal , group b , b cell , microbiology and biotechnology , group a , reagent , phenotype , antigen , biology , chemistry , immunology , antibody , medicine , genetics , gene
SUMMARY. During the period 1984–1989, a total of 46 examples of B v phenotype were encountered out of a total of 567,210 donors, giving an incidence of 1 in 12,330 among the Chinese in Hong Kong. The B v determinant corresponds to the portion of the B antigen that is present on rabbit red cells, and gives a negative reaction with polyclonal anti‐B reagents absorbed with rabbit red cells that still react with B 3 . Some potent monoclonal anti‐B reagents confirm the absence of a B epitope from B v red cells even by adsorption and elution techniques. The failure of some monoclonal anti‐B reagents to detect B v demonstrates the need to select or blend monoclonal anti‐B reagents for use in typing Oriental bloods. Cell‐conversion techniques failed to convert O cells to B cells using B v serum with the appropriate substrate, whereas sera from most of the other B variants were capable of doing so. The B v phenotype, therefore, represents a distinct category of B subgroups that is easily distinguishable from B 3 and other B variants.