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Is there a wastage of resources due to non‐specificity of anti‐HIV ELISAs?
Author(s) -
Rodriguez M. A.,
Runganga J.,
Contreras M.,
Ahya R.,
Barbara J. A. J.
Publication year - 1991
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.1365-3148.1991.tb00044.x
Subject(s) - human immunodeficiency virus (hiv) , medicine , false positive paradox , virology , artificial intelligence , computer science
Summary. A library of anti‐HIV ELISA ‘grey‐area’ and repeatably reactive samples sent for confirmatory testing, were retested using a second technique, the modified (Fujirebio Gelatin Particle) agglutination test (MAT). On testing 224 grey‐area reactive samples, only four were found to be reactive with this second test. On retesting a further 259 ELISA repeatably reactive samples, of which only 33 were confirmed to be anti‐HIV‐1, only 45 were reactive by MAT; these included the 33 confirmed as positive samples, thereby reducing the false‐positives from 226 to 12. The introduction of this second technique supported our decision to cease the referral of grey‐area samples. It also demonstrated the high prevalence of non‐specificity of repeatable reactivity associated with some of the most specific ELISA kits currently available.