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Quantification of IgG on erythrocytes of patients and normals by a radio‐ligand‐binding assay
Author(s) -
Giles C. M.,
Davies K. A.,
Loizou S.,
Moulds J. J.,
Walport M. J.
Publication year - 1991
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.1365-3148.1991.tb00037.x
Subject(s) - agglutination (biology) , ligand binding assay , monoclonal antibody , microbiology and biotechnology , immunology , chemistry , immune system , antibody , ligand (biochemistry) , monoclonal , lupus erythematosus , immune complex , immunoglobulin g , medicine , biology , biochemistry , receptor
Summary. A monoclonal IgG anti‐human IgG, 1B12, was used in a radio‐ligand‐binding assay to quantify IgG on erythrocytes of patients and normals. The assay detected a range of 10–700 IgG molecules. Good correlation was achieved between the number of molecules and the strength of agglutination in antiglobulin tests performed in capillary tubes. The assay was capable of detecting subagglutinating immune bound IgG on erythrocytes from patients with systemic lupus erythematosus (SLE).