Premium
The effect of intravenous immunoglobulin on placental transfer of a platelet‐specific antibody: Anti‐P1 A1
Author(s) -
Morgan C. L.,
Cannell G. R.,
Addison R. S.,
Minchinton R. M.
Publication year - 1991
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1111/j.1365-3148.1991.tb00035.x
Subject(s) - antibody , placenta , fetal circulation , fetus , platelet , immunoglobulin g , immunology , in vitro , chemistry , andrology , pregnancy , biology , medicine , biochemistry , genetics
Summary. The isolated perfused lobule of human placenta was used as an in‐vitro model to study the effect of intravenous immunoglobulin (IVGG) on the placental transfer of a human platelet‐specific antibody (anti‐P1 A1 ). Normal human IgG was shown to transfer from the maternal to the fetal circulation of the placental model after a lag period of 2–3 h. IVGG also transferred across the placenta but only after a longer lag period (3–4 h) than normal human IgG at the same concentration, which suggests that IVGG may contain a factor that inhibits the transfer of its own component IgG. The sensitive Western immunoblotting technique was used to demonstrate progressive transfer of anti‐P1 A1 antibody to the fetal circulation after a 2–3 h lag period. When IVGG and anti‐P1 A1 antibody were added simultaneously to the maternal circulation, the transfer of platelet‐specific antibody was strongly inhibited by IVGG. The inhibitory effect of IVGG on anti‐P1 A1 antibody transfer was consistent for three different batches of the same IVGG product (Sandoglobulin). These studies provide the first scientific data to support the use of IVGG to inhibit antiplatelet antibody transfer as part of the antenatal management of neonatal alloimmune thrombocytopenia.