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Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Author(s) -
Xu Deyang,
Huang Weihua,
Li Yang,
Wang Hua,
Huang Hai,
Cui Xiaofeng
Publication year - 2012
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2011.04831.x
Subject(s) - biology , microbiology and biotechnology , cell cycle , chromatin , dna replication factor cdt1 , eukaryotic dna replication , mitosis , origin recognition complex , proliferating cell nuclear antigen , dna replication , control of chromosome duplication , histone , licensing factor , genetics , cell growth , cell , dna
Summary The mitotic cell cycle in higher eukaryotes is of pivotal importance for organ growth and development. Here, we report that Elongator, an evolutionarily conserved histone acetyltransferase complex, acts as an important regulator of mitotic cell cycle to promote leaf patterning in Arabidopsis. Mutations in genes encoding Elongator subunits resulted in aberrant cell cycle progression, and the altered cell division affects leaf polarity formation. The defective cell cycle progression is caused by aberrant DNA replication and increased DNA damage, which activate the DNA replication checkpoint to arrest the cell cycle. Elongator interacts with proliferating cell nuclear antigen (PCNA) and is required for efficient histone 3 (H3) and H4 acetylation coupled with DNA replication. Levels of chromatin‐bound H3K56Ac and H4K5Ac known to associate with replicons during DNA replication were reduced in the mutants of both Elongator and chromatin assembly factor 1 (CAF‐1), another protein complex that physically interacts with PCNA for DNA replication‐coupled chromatin assembly. Disruptions of CAF‐1 also led to severe leaf polarity defects, which indicated that Elongator and CAF‐1 act, at least partially, in the same pathway to promote cell cycle progression. Collectively, our results demonstrate that Elongator is an important regulator of mitotic cell cycle, and the Elongator pathway plays critical roles in promoting leaf polarity formation.