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Establishment of a Lotus japonicus gene tagging population using the exon‐targeting endogenous retrotransposon LORE1
Author(s) -
Fukai Eigo,
Soyano Takashi,
Umehara Yosuke,
Nakayama Shinobu,
Hirakawa Hideki,
Tabata Satoshi,
Sato Shusei,
Hayashi Makoto
Publication year - 2012
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2011.04826.x
Subject(s) - retrotransposon , biology , genetics , lotus japonicus , population , gene , long terminal repeat , genome , endogenous retrovirus , mutant , computational biology , transposable element , demography , sociology
Summary We established a gene tagging population of the model legume Lotus japonicus using an endogenous long terminal repeat (LTR) retrotransposon Lotus Retrotransposon 1 ( LORE1 ). The population was composed of 2450 plant lines, from which a total of 4532 flanking sequence tags of LORE1 were recovered by pyrosequencing. The two‐dimensional arrangement of the plant population, together with the use of multiple identifier sequences in the primers used to amplify the flanking regions, made it possible to trace insertions back to the original plant lines. The large‐scale detection of new LORE1 insertion sites revealed a preference for genic regions, especially in exons of protein‐coding genes, which is an interesting feature to consider in the interaction between host genomes and chromoviruses, to which LORE1 belongs, a class of retrotransposon widely distributed among plants. Forward screening of the symbiotic mutants from the population succeeded to identify five symbiotic mutants of known genes. These data suggest that LORE1 is robust as a genetic tool.