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A leucine‐rich repeat receptor‐like kinase gene is involved in the specification of outer cell layers in rice roots
Author(s) -
Huang ChaoFeng,
Yamaji Naoki,
Ono Kazuko,
Ma Jian Feng
Publication year - 2012
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2011.04824.x
Subject(s) - biology , mutant , endodermis , gene , microbiology and biotechnology , root hair , subcellular localization , cell wall , epidermis (zoology) , cell , oryza sativa , root cap , genetics , meristem , anatomy
Summary Root outer cell layers of Oryza sativa (rice), which comprise the epidermis, exodermis and sclerenchyma, play an important role in protecting the roots from various stresses in soil, but the molecular mechanisms for the specification of these cell layers are poorly understood. In this work, we report on defective in outer cell layer specification 1 ( Docs1 ), which is involved in the specification of outer cell layers in rice roots. Docs1 was isolated by map‐based cloning using a mutant ( c68 ) defective in the outer cell layers of primary roots. It encodes a leucine‐rich repeat receptor‐like kinase (LRR RLK). Docs1 mRNA was expressed in all tissues including roots, leaf blades and sheaths, and flowers. Immunostaining with an anti‐Docs1 antibody showed that Docs1 was localized at the epidermis and exodermis, depending on the root region. Furthermore, Docs1 showed polar localization at the distal side. Subcellular examination showed that Docs1 was localized to the plasma membrane. Comparison of genome‐wide transcriptional profiles between the wild‐type and the knock‐out mutant roots using microarray analysis showed that 61 and 41 genes were up‐ and downregulated in the mutant, including genes encoding putative transcription factors and genes potentially involved in cell wall metabolism. These results suggest that Docs1 might directly or indirectly regulate multiple genes involved in the proper development of root outer cell layers in rice.