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Suppression of the rice heterotrimeric G protein β‐subunit gene, RGB1 , causes dwarfism and browning of internodes and lamina joint regions
Author(s) -
Utsunomiya Yuzuko,
Samejima Chihiro,
Takayanagi Yoshiyuki,
Izawa Yuki,
Yoshida Takahisa,
Sawada Yuka,
Fujisawa Yukiko,
Kato Hisaharu,
Iwasaki Yukimoto
Publication year - 2011
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2011.04643.x
Subject(s) - heterotrimeric g protein , biology , protein subunit , microbiology and biotechnology , gene , mutant , g alpha subunit , wild type , g protein , genetics , signal transduction
Summary In the present study, we investigated the function of the heterotrimeric G protein β‐subunit (Gβ) gene ( RGB1 ) in rice. RGB1 knock‐down lines were generated in the wild type and d1‐5 , a mutant deficient for the heterotrimeric G protein α‐subunit (Gα) gene ( RGA1 ). Both transgenic lines showed browning of the lamina joint regions and nodes that could be attributed to a reduction of RGB1 function, as the abnormality was not observed in d1‐5 . The RGB1 knock‐down lines generated in d1‐5 were shorter, suggesting RGB1 to be a positive regulator of cellular proliferation, in addition to RGA1 . The number of sterile seeds also increased in both RGB1 knock‐down lines. These results suggest that Gβγ and Gα cooperatively function in cellular proliferation and seed fertility. We discuss the potential predominant role of RGB1 in G protein signaling in rice.