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Functional characterization of the CKRC1/TAA1 gene and dissection of hormonal actions in the Arabidopsis root
Author(s) -
Zhou ZhaoYang,
Zhang ChunGuang,
Wu Lei,
Zhang CaiGuo,
Chai Juan,
Wang Ming,
Jha Ajay,
Jia PengFei,
Cui SuJuan,
Yang Ming,
Chen Rujin,
Guo GuangQin
Publication year - 2011
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2011.04509.x
Subject(s) - auxin , arabidopsis , mutant , cytokinin , plant hormone , polar auxin transport , biology , microbiology and biotechnology , biosynthesis , lateral root , biochemistry , gene , chemistry
Summary Cytokinin (CK) influences many aspects of plant growth and development, and its function often involves intricate interactions with other phytohormones such as auxin and ethylene. However, the molecular mechanisms underlying the role of CK and its interactions with other growth regulators are still poorly understood. Here we describe the isolation and characterization of the Arabidopsis CK‐induced root curling 1 ( ckrc1 ) mutant. CKRC1 encodes a previously identified tryptophan aminotransferase (TAA1) involved in the indole‐3‐pyruvic acid (IPA) pathway of indole‐3‐acetic acid (IAA) biosynthesis. The ckrc1 mutant exhibits a defective root gravitropic response (GR) and an increased resistance to CK in primary root growth. These defects can be rescued by exogenous auxin or IPA. Furthermore, we show that CK up‐regulates CKRC1/TAA1 expression but inhibits polar auxin transport in roots in an AHK3/ARR1/12‐dependent and ethylene‐independent manner. Our results suggest that CK regulates root growth and development not only by down‐regulating polar auxin transport, but also by stimulating local auxin biosynthesis.