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The PAM1 gene of petunia, required for intracellular accommodation and morphogenesis of arbuscular mycorrhizal fungi, encodes a homologue of VAPYRIN
Author(s) -
Feddermann Nadja,
Duvvuru Muni Rajasekhara Reddy,
Zeier Tatyana,
Stuurman Jeroen,
Ercolin Flavia,
Schorderet Martine,
Reinhardt Didier
Publication year - 2010
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2010.04341.x
Subject(s) - biology , symbiosis , hypha , intracellular , botany , microbiology and biotechnology , petunia , morphogenesis , mutant , cullin , lotus japonicus , cytosol , gene , genetics , bacteria , ubiquitin ligase , biochemistry , ubiquitin , enzyme
Summary Most terrestrial plants engage into arbuscular mycorrhizal (AM) symbiosis with fungi of the phylum Glomeromycota . The initial recognition of the fungal symbiont results in the activation of a symbiosis signalling pathway that is shared with the root nodule symbiosis (common SYM pathway). The subsequent intracellular accommodation of the fungus, and the elaboration of its characteristic feeding structures, the arbuscules, depends on a genetic programme in the plant that has recently been shown to involve the VAPYRIN gene in Medicaco truncatula . We have previously identified a mutant in Petunia hybrida , penetration and arbuscule morphogenesis 1 ( pam1 ), that is defective in the intracellular stages of AM development. Here, we report on the cloning of PAM1 , which encodes a VAPYRIN homologue. PAM1 protein localizes to the cytosol and the nucleus, with a prominent affinity to mobile spherical structures that are associated with the tonoplast, and are therefore referred to as tonospheres. In mycorrhizal roots, tonospheres were observed in the vicinity of intracellular hyphae, where they may play an essential role in the accommodation and morphogenesis of the fungal endosymbiont.