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The AAA‐type ATPase AtSKD1 contributes to vacuolar maintenance of Arabidopsis thaliana
Author(s) -
Shahriari Mojgan,
Keshavaiah Channa,
Scheuring David,
Sabovljevic Aneta,
Pimpl Peter,
Häusler Rainer E.,
Hülskamp Martin,
Schellmann Swen
Publication year - 2010
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2010.04310.x
Subject(s) - vacuole , trichome , microbiology and biotechnology , arabidopsis , biology , arabidopsis thaliana , v atpase , vacuolar protein sorting , biogenesis , secretion , organelle , atpase , plant cell , botany , biochemistry , gene , cytoplasm , mutant , enzyme
Summary The vacuole is the most prominent organelle of plant cells. Despite its importance for many physiological and developmental aspects of plant life, little is known about its biogenesis and maintenance. Here we show that Arabidopsis plants expressing a dominant‐negative version of the AAA (ATPase associated with various cellular activities) ATPase AtSKD1 (SUPPRESSOR OF K + TRANSPORT GROWTH DEFECT1) under the control of the trichome‐specific GLABRA2 (GL2) promoter exhibit normal vacuolar development in early stages of trichome development. Shortly after its formation, however, the large central vacuole is fragmented and finally disappears completely. Secretion assays with amylase fused to the vacuolar sorting signal of Sporamin show that dominant‐negative AtSKD1 inhibits vacuolar trafficking of the reporter that is instead secreted. In addition, trichomes expressing dominant‐negative AtSKD1 frequently contain multiple nuclei. Our results suggest that AtSKD1 contributes to vacuolar protein trafficking and thereby to the maintenance of the large central vacuole of plant cells, and might play a role in cell‐cycle regulation.