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Tobacco mutants with reduced microtubule dynamics are less susceptible to TMV
Author(s) -
Ouko Maurice O.,
Sambade Adrian,
Brandner Katrin,
Niehl Annette,
Peña Eduardo,
Ahad Abdul,
Heinlein Manfred,
Nick Peter
Publication year - 2010
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2010.04195.x
Subject(s) - tobacco mosaic virus , microtubule , mutant , green fluorescent protein , wild type , movement protein , tubulin , tobamovirus , biology , microbiology and biotechnology , virology , chemistry , virus , genetics , gene , rna , coat protein
Summary A panel of seven SR1 tobacco mutants ( ATER1 to ATER7 ) derived via T‐DNA activation tagging and screening for resistance to a microtubule assembly inhibitor, ethyl phenyl carbamate, were used to study the role of microtubules during infection and spread of tobacco mosaic virus (TMV). In one of these lines, ATER2 , α‐tubulin is shifted from the tyrosinylated into the detyrosinated form, and the microtubule plus‐end marker GFP–EB1 moves significantly slower when expressed in the background of the ATER2 mutant as compared with the SR1 wild type. The efficiency of cell‐to‐cell movement of TMV encoding GFP‐tagged movement protein (MP‐GFP) is reduced in ATER2 accompanied by a reduced association of MP‐GFP with plasmodesmata. This mutant is also more tolerant to viral infection as compared with the SR1 wild type, implying that reduced microtubule dynamics confer a comparative advantage in face of TMV infection.