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BODIPY probes to study peroxisome dynamics in vivo
Author(s) -
Landrum Marie,
Smertenko Andrei,
Edwards Robert,
Hussey Patrick J.,
Steel Patrick G.
Publication year - 2010
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2010.04153.x
Subject(s) - peroxisome , bodipy , biology , fluorescence , in vivo , biomolecule , plant cell , microbiology and biotechnology , biochemistry , biophysics , cell , function (biology) , computational biology , gene , genetics , physics , quantum mechanics
Summary There is a continuing need for bioprobes that are target‐specific and combine speed of delivery with maintenance of normal cell behaviour. Towards this end, we are developing small pro‐fluorescent molecules that provide such specificity through chemical activation by biomolecules. We have generated a set of BODIPY (boron dipyrromethane) fluorophores, including one that is intrinsically non‐fluorescent but on incubation with cells becomes fluorescent at its target site. Addition of these BODIPY probes to plant cells identifies peroxisomes, as verified by co‐localization with an SKL‐FP construct. Interestingly, in mammalian cells, co‐localization with the mammalian peroxisomal marker SelectFX TM was not observed. These data suggest fundamental differences in peroxisome composition, development or function between plant and animal cells.